Introduction to Fluorescence Imaging for the Analysis of Living Cells
Introductory course given by Professor Jean-Yves CHATTON, Cellular Imaging Facility (CIF) – 2020
Time and location
Place: Petit Auditoire de l’Ecole de Médecine, rue du Bugnon 9 (1er étage)
Schedule: Winter Semester – Tuesdays (mostly) 12:15 – 14:00
Schedule
- 7 January 2020: Basics of transmitted light and fluorescence microscopy
- 14 January 2020: Confocal microscopy
- 21 January 2020: Modes of image formation, acquisition, signal sampling
- 28 January 2020: Dynamic recording of cellular functions by fluorescence imaging. Intracellular ion imaging and cellular signaling. Problems related to imaging of living cells
- 4 February 2020: Other optical applications (proposed topics) Fluorescence recovery after photobleaching (FRAP) , photoactivation-optogenetics, multiphoton microscopy, fluorescence resonance energy transfer (FRET), optical contrasting methods (DIC, phase, …), Super-resolution microscopy.
Remarks
- Lectures will be given in English.
- In parallel with theoretical lectures, demonstrations will be proposed on the confocal microscopes of the Cellular Imaging Facility (details and registration on site).
- Gives right to ONE credit for students of doctoral schools (FBM and neuroscience)
Admission to the course is free and open to anyone interested.
Please register to jean-yves.chatton@unil.ch — on site registration possible