Rue du Bugnon 9, CH-1005, Lausanne, VD, Switzerland
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CIF Coordinator:


Introduction to Fluorescence Imaging for the Analysis of Living Cells

Introductory course given by Professor Jean-Yves CHATTON, Cellular Imaging Facility (CIF) – 2020

Time and location

Place: Petit Auditoire de l’Ecole de Médecine, rue du Bugnon 9 (1er étage)
Schedule: Winter Semester – Tuesdays (mostly) 12:15 – 14:00


  • 7 January 2020: Basics of transmitted light and fluorescence microscopy
  • 14 January 2020: Confocal microscopy
  • 21 January 2020: Modes of image formation, acquisition, signal sampling
  • 28 January 2020: Dynamic recording of cellular functions by fluorescence imaging. Intracellular ion imaging and cellular signaling. Problems related to imaging of living cells
  • 4 February 2020: Other optical applications (proposed topics) Fluorescence recovery after photobleaching (FRAP) , photoactivation-optogenetics, multiphoton microscopy, fluorescence resonance energy transfer (FRET), optical contrasting methods (DIC, phase, …), Super-resolution microscopy.


  • Lectures will be given in English.
  • In parallel with theoretical lectures, demonstrations will be proposed on the confocal microscopes of the Cellular Imaging Facility (details and registration on site).
  • Gives right to ONE credit for students of doctoral schools (FBM and neuroscience)

Admission to the course is free and open to anyone interested.
Please register  to  — on site registration possible