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Contact : supportcif@unil.ch

Agora Instruments

Acquisition systems

Confocal

Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.[1] Capturing multiple two-dimensional images at different depths in a sample enables the reconstruction of three-dimensional structures (a process known as optical sectioning) within an object.

Olympus FluoView 3000

Illumination

  • LED; pE-300white; CoolLED

Camera

Olympus DP80

Sensor:CCD color
Number of pixels:4080 x 3072
Chip area: 8.8 x 6.6 mm
Pixel chip size: 6.45 x 6.45 µm
Dynamic range: 42 bit (colour)

Lasers

  • 405nm

  • 488nm

  • 561nm

  • 640nm

Objectives

  • Plan Apochromat 1.25x/0.04 WD 5.0mm no contrast dry

  • Plan Apochromat 10x/0.4 WD 3.1mm DIC dry

  • Plan Apochromat 20x/0.75 WD 0.6mm DIC dry

  • Plan Fluorite 40x/1.3 WD 0.2mm DIC oil

  • Plan Apochromat 60x/1.42 WD 0.15mm DIC oil

Stage

  • Motorized

Contrast

  • DIC

CO2 | T° control

  • No

Detector type

  • 1x T-PMT

  • 4x GaAsP

Optional modules

 

Software

  • CellSens

  • FluoView 31S-SW

Room

  • B25A.03.201

Super Resolution Microscopy

Super resolution microscopy goes beyond the limitations of conventional microscopy by allowing researchers to visualize intricate details at the sub-organelle level. The microscope can incorporate advanced techniques such as Lattice SIM², SIM² Apotome, widefield DIC, SMLM, and TIRF, enabling the correlation of images from the same sample to reveal molecular details with exceptional clarity and precision. This cutting-edge technology provides insights into cellular structures and processes at scales previously unattainable, making it a powerful tool for biological research across various applications.

Zeiss Elyra 7

Model

  • Zeiss Axio Observer 7 SR RP stand for Elyra 7, Inverted

Widefield Illumination

  • HXP 120 V

Lasers

  • 405 nm (50 mW)
  • 488 nm (500mW)
  • 561 nm (500 mW)
  • 642 nm (500 mW)

Filters

  • DAPI (BP 420-480)
  • GFP (BP 495-550)
  • Rhodamin (BP 570-620)
  • Cy5 (LP 655)

Objectives

  • Plan Neofluar 10x / NA 0.30 / dry /WD 5.2mm
  • Plan Apochromat 20x / NA 0.8 / dry / WD 0.55mm
  • Plan Apochromat 40x / NA 1.4 / oil / WD 0.13mm
  • Plan Apochromat 63x / NA 1.4 / oil / WD 0.19mm
  • α Plan Apochromat 63x TIRF / NA 1.46 / oil / WD 0.10mm

Stage

  • Motorized
  • Piezo (Z direction)
  • Definite Focus 3

Contrast

  • No

CO2 | T° control

  • Yes both

Incubation

  • Full enclosure / Dark

Cameras

  • 2x PCO Edge sCMOS DuoLink (simultaneous two-color Imaging)

Imaging Modalities

  • Widefield
  • Lattice SIM2
  • Single-Molecule Localization Microscopy
  • Apotome
  • HILO/TIRF

Software

  • Zeiss Zen Black 3.0 SR

Room

  • B25A.03.201

Spinning Disk | Time-Lapse

Spinning-disk (Nipkow disk) confocal microscopes use a series of moving pinholes on a disc to scan spots of light. Since a series of pinholes scans an area in parallel, each pinhole is allowed to hover over a specific area for a longer amount of time thereby reducing the excitation energy needed to illuminate a sample when compared to laser scanning microscopes. Decreased excitation energy reduces phototoxicity and photobleaching of a sample often making it the preferred system for imaging live cells or organisms.

Nikon Ti2 | Yokogawa CSU-W1

Widefield LED Illumination

Lumencore Spectra X LED

  • 395nm

  • 440nm

  • 470nm

  • 508nm

  • 555nm

  • 640nm

Filter Cubes

  • Quintuple multiband filtercube for DAPI/FITC/TRITC/Cy5/Cy7 (32mm)
  • DAPI-5060C Filter Cube (32mm)
  • GFP-4050B Filter Cube (32mm)
  • Cy3-4040C Filter Cube (32mm)
  • Cy5-4040C Filter Cube (32mm)

Spinning Disk laser excitation

Omicron modified LightHUB+

  • 405nm
  • 488nm

  • 561nm
  • 638nm

Objectives

  • CFI Plan Apochromat Lambda 10X, N.A. 0.45, W.D. 4.0mm
  • CFI Plan Apochromat Lambda 20X, N.A. 0.75, W.D. 1.0mm, spring-loaded
  • CFI Plan Fluorite 40X, N.A. 0.75, W.D. 0.66mm
  • CFI Plan Apochromat Lambda 60x, N.A. 0.95, W.D. 0.21mm, Spring-loaded
  • CFI Plan Apochromat Lambda 60X Oil, N.A. 1.40, W.D. 0.13mm
  • CFI Plan Fluorite 100X Oil, N.A. 1.30, W.D. 0.16mm

Stage

  • Motorized

  • Piezo (Z direction)
  • Nikon PFS (Perfect Focus System)

Contrast

  • DIC

CO2 | T° control

  • Yes [Full enclosure]

Cameras

  • 2x Photometrics Prime 95B

  • 1x Nikon DS-Qi2

Optional modules

  • JOBS
  • General Analysis 3
  • Deconvolution

Software

  • Nikon NIS-Elements AR 5.0

Room

  • B25A.03.201

Widefield | Fluorescence

The majority of fluorescence microscopes, especially those used in the life sciences, are of the epifluorescence design shown in the diagram. Light of the excitation wavelength illuminates the specimen through the objective lens. The fluorescence emitted by the specimen is focused to the detector by the same objective that is used for the excitation which for greater resolution will need objective lens with higher numerical aperture. Since most of the excitation light is transmitted through the specimen, only reflected excitatory light reaches the objective together with the emitted light and the epifluorescence method therefore gives a high signal-to-noise ratio. The dichroic beamsplitter acts as a wavelength specific filter, transmitting fluoresced light through to the eyepiece or detector, but reflecting any remaining excitation light back towards the source.

Zeiss | Widefield

 

Stage
  • Zeiss Axio Observer Z1, Inverted

Illumination

  • X-Cite

Filter Cubes

  • DAPI
  • GFP
  • Rhodamin
  • Cy5

Objectives

  • Plan Apochromat 10X, N.A. 0.45, air, DIC, W.D. 2.0mm
  • Plan Apochromat 20X, N.A. 0.8, air, DIC, W.D. 0.55mm
  • EC Plan Neofluar 40X, N.A. 1.3, Oil, DIC, W.D. 0.21mm
  • Plan Apochromat 63X, N.A. 1.4, Oil, DIC, W.D. 0.19mm

Stage

  • Motorized

Contrast

  • DIC

CO2 | T° control

  • No | No

Cameras

  • Axiocam 506 color
  • Axiocam 506 mono

Optional modules

  • Tile (Image stitching)

Software

  • Zeiss Zen 2.6 Blue edition

Room

  • B25A.03.201

Slide scanner

A slide scanner allows you to digitize full microscopy slides in an automated manner. This is a high througput system able to process around a hundred of slides in one batch.

Zeiss Axioscan 7

Illumination

Zeiss Colibri 7 Type R[G/Y]CBV-UV

  • Far Red (735nm) for excitation of Alexa Fluor 750, Cy7 and similar dyes

  • Red (631nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes

  • Yellow (590nm) for excitation of mCherry, Alexa 568, mPlum and similar dyes

  • Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes

  • (Cyan (511nm) for excitation of eYFP, Eosin, TOTO-1 and similar dyes) no filters available

  • Blue (469nm) for excitation of eGFP, Fluo4, FITC and similar dyes

  • (Violet (423nm) for excitation of eCFP, Lucifer Yellow, Alexa 430 and similar dyes) no filters available

  • UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes

Filters

  • BFP: EX 390/40, DM 420, EM 450/40, 96 HE BFP shift free

  • eGFP: 470/40, 495, 525/50, 38 HE eGFP shift free

  • Cy3: 550/25, 570, 605/70, 43 HE Cy3 shift free

  • mPlum: 587/25, 605, 647/70, 64 HE mPlum shift free

  • Cy5: 640/30, 660, 690/50, 50 Cy5 shift free

  • Cy7: 734/40, 762, 785/38, 110 HE LED

Objectives

  • Fluar 5x/0.25
  • Plan Apochromat 10x/0.45

  • Plan Apochromat 20x/0.80

  • Plan Apochromat 40x/0.95

Stage

  • Motorized

Contrast

  • Brightfield, Transfer of Intensity Equation (TIE), Fluorescence

CO2 | T° control

  • No

Cameras

Color camera

  • Zeiss Axiocam 705 for Axio Scan 7

Black and White Camera

  • Orca Flash 4.0 V3 for Axio Scan 7

Optional modules

  • NC

Software

  • Zen Blue 3.5

Room

  • B25A.03.201

Image Processing

[Under construction]